Applications of Biotechnology Practice Test

12th Standard Zoology — Applications of Biotechnology: Online Practice Test

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The first clinical gene therapy was done for the treatment of

A.AIDS B.Cancer C.Cystic fibrosis D.SCID

Dolly, the sheep was obtained by a technique known as

A.Cloning by gene transfer B.Cloning without the help of gametes C.Cloning by tissue culture of somatic cells D.Cloning by nuclear transfer

The genetic defect adenosine deaminase deficiency may be cured permanently by

A.Enzyme replacement therapy B.Periodic infusion of genetically engineered lymphocytes having ADA cDNA C.Administering adenosine deaminase activators D.Introducing bone marrow cells producing ADA into embryo at an early stage of development

How many amino acids are arranged in the two chains of Insulin?

A.Chain A has 12 and Chain B has 13 amino acids B.Chain A has 21 and Chain B has 30 amino acids C.Chain A has 20 and chain B has 30 amino acids D.Chain A has 12 and chain B has 20 amino acids

PCR proceeds in three distinct steps governed by temperature, they are in order of

A.Denaturation, Annealing, Synthesis B.Synthesis, Annealing, Denaturation C.Annealing, Synthesis, Denaturation D.Denaturation, Synthesis, Annealing

Which one of the following statements is true regarding DNA polymerase used in PCR?

A.It is used to ligate introduced DNA in recipient cells B.It serves as a selectable marker C.It is isolated from a Virus D.It remains active at a high temperature

ELISA is mainly used for

A.Detection of mutations B.Detection of pathogens C.Selecting animals having desired traits D.Selecting plants having desired traits

Transgenic animals are those which have

A.Foreign DNA in some of their cells B.Foreign DNA in all their cells C.Foreign RNA in some of their cells D.Foreign RNA in all their cells

Vaccines that use components of a pathogenic organism rather than the whole organism are called

A.Subunit recombinant vaccines B.attenuated recombinant vaccines C.DNA vaccines D.conventional vaccines

Q10

GEAC stands for

A.Genome Engineering Action Committee B.Ground Environment Action Committee C.Genetic Engineering Approval Committee D.Genetic and Environment Approval Committee

Q11

Under which trade name was the first recombinant human insulin commercially marketed in the year 1986?

A.Humulin B.Insulin-G C.Recombivax D.Engerix-B

Q12

What is the molecular weight of the human alpha-lactalbumin protein, which is found abundantly in human milk and has been expressed in transgenic cows?

A.14178 Da B.12110 Da C.24000 Da D.51000 Da

Q13

Why is the yeast Saccharomyces cerevisiae preferred over Escherichia coli for the commercial production of recombinant interferons?

A.E. coli does not possess the genetic machinery required for the glycosylation of proteins. B.Saccharomyces cerevisiae grows much faster than E. coli in fermentation tanks. C.E. coli is highly sensitive to antiviral substances like interferons. D.Saccharomyces cerevisiae has a smaller genome size than E. coli.

Q14

Which country, after the USA, France, and Belgium, was the fourth in the world to develop an indigenous recombinant vaccine for hepatitis B?

A.India B.Germany C.United Kingdom D.Canada

Q15

What genetic mechanism is utilized in gene inhibition therapy to prevent the expression of a dominant mutant gene?

A.Insertion of a functional wild-type allele to boost the missing product B.Insertion of an antisense gene that blocks the translation of the dominant gene's mRNA C.Excision of the mutant promoter using restriction endonucleases D.Direct deletion of the dominant mutated exon in mature somatic cells

Q16

Which level of cellular potency is exhibited by lymphoid or myeloid stem cells, which can differentiate into a few specific cell types like B and T cells but not red blood cells?

A.Pluripotency B.Multipotency C.Oligopotency D.Unipotency

Q17

Who discovered the Enzyme-Linked Immunosorbent Assay (ELISA) technique in 1971?

A.Eva Engvall and Peter Perlmann B.Ian Wilmut and Keith Campbell C.Alick Isaacs and Jean Lindemann D.Kary Mullis and Alec Jeffreys

Q18

During reverse transcription PCR (RT-PCR), which enzyme is initially required to synthesize complementary DNA (cDNA) from a messenger RNA (mRNA) template?

A.Taq DNA polymerase B.Reverse transcriptase C.DNA ligase D.Ribonuclease H

Q19

What term is used to describe a genetically engineered organism in which one or more specific genes on its chromosomes have been deliberately made non-functional or inoperative?

A.Transgene carrier B.Gene knock out C.Somatic hybrid D.Autosomal aneuploid

Q20

In the cloning of Dolly the sheep, how many reconstructed embryos were actually implanted into surrogate mothers after six days of culture?

A.277 B.29 C.5 D.12

Q21

Which Indian statutory committee under the Department of Biotechnology (DBT) is specifically responsible for issuing permits for research involving genetically engineered organisms?

A.Genetic Engineering Approval Committee (GEAC) B.Review Committee on Genetic Manipulation (RCGM) C.Institutional Biosafety Committee (IBSC) D.Recombinant DNA Advisory Committee (RDAC)

Q22

Which was the first living organism ever to be patented, following its genetic engineering in 1971 by scientist Ananda Mohan Chakrabarty?

A.Escherichia coli B.Saccharomyces cerevisiae C.Pseudomonas putida D.Trichoderma polysporum

Q23

What term is used to describe the exploitation of biological resources by multinational companies without proper authorization or compensatory payment to the source country or its people?

A.Biopiracy B.Bioethics C.Bioprospecting D.Patent infringement

Q24

Which of the following products from Tamil Nadu is specifically mentioned in the textbook as having received a Geographical Indication (GI) tag?

A.Kancheepuram silk B.Tirunelveli halwa C.Salem mango D.Nilgiri tea

Q25

For how many years does a government-issued patent typically grant an inventor the exclusive right to manufacture, use, or sell their biotechnological invention?

A.10 years B.20 years C.30 years D.50 years

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About this Applications of Biotechnology test

This free online practice test covers Applications of Biotechnology from the 12th Standard Zoology (Samacheer Kalvi) syllabus. Choose the number of questions and an optional time limit, then answer and submit — everything is checked in your browser, with the correct answers and a worked explanation shown at the end. For the full solutions to every question in this set, see the solved MCQs page.

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